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imagelab analytic software  (Bio-Rad)


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    Bio-Rad imagelab analytic software
    Imagelab Analytic Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/analytical+imaging+software/Image+Lab+Software/pmc12513116-185-0-4
    Average 99 stars, based on 35737 article reviews
    imagelab analytic software - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Targeting the TCA cycle through cuproptosis confers synthetic lethality on ARID1A-deficient hepatocellular carcinoma.
    Article Snippet: Lysis buffer (KeyGen BioTECH; #KGP250) was used to extract total protein from cells on ice, after which these lysates were separated via 12.5% SDS‒PAGE (70 min, 120 V) and transferred on ice onto PVDF membranes (Merck Millipore; #IPVH00010) using transfer buffer containing 10–20%methanol (1–2.5 h, 300 mA). .. The membranes were blocked using 5% nonfat milk prior to incubation with appropriate primary and secondary antibodies and imaging with a chemiluminescent substrate (Merck Millipore; #WBKLS0100) and Bio-Rad Gel Doc 2000 analytical software. .. The membranes were then stripped with Western Blot Fast Stripping Buffer (EpiZyme; # PS107) and incubated with the next antibodies as appropriate.

    Imaging:

    Article Title: Targeting the TCA cycle through cuproptosis confers synthetic lethality on ARID1A-deficient hepatocellular carcinoma.
    Article Snippet: Lysis buffer (KeyGen BioTECH; #KGP250) was used to extract total protein from cells on ice, after which these lysates were separated via 12.5% SDS‒PAGE (70 min, 120 V) and transferred on ice onto PVDF membranes (Merck Millipore; #IPVH00010) using transfer buffer containing 10–20%methanol (1–2.5 h, 300 mA). .. The membranes were blocked using 5% nonfat milk prior to incubation with appropriate primary and secondary antibodies and imaging with a chemiluminescent substrate (Merck Millipore; #WBKLS0100) and Bio-Rad Gel Doc 2000 analytical software. .. The membranes were then stripped with Western Blot Fast Stripping Buffer (EpiZyme; # PS107) and incubated with the next antibodies as appropriate.

    Software:

    Article Title: Targeting the TCA cycle through cuproptosis confers synthetic lethality on ARID1A-deficient hepatocellular carcinoma.
    Article Snippet: Lysis buffer (KeyGen BioTECH; #KGP250) was used to extract total protein from cells on ice, after which these lysates were separated via 12.5% SDS‒PAGE (70 min, 120 V) and transferred on ice onto PVDF membranes (Merck Millipore; #IPVH00010) using transfer buffer containing 10–20%methanol (1–2.5 h, 300 mA). .. The membranes were blocked using 5% nonfat milk prior to incubation with appropriate primary and secondary antibodies and imaging with a chemiluminescent substrate (Merck Millipore; #WBKLS0100) and Bio-Rad Gel Doc 2000 analytical software. .. The membranes were then stripped with Western Blot Fast Stripping Buffer (EpiZyme; # PS107) and incubated with the next antibodies as appropriate.



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    Image Search Results


    Gene expression analysis of OCLN and markers related to its trafficking in WT and CB1 −/− (KO) testes. RTqPCR analysis of Ocln mRNA. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (A). Western blot analysis of OCLN. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD values (B). Immunofluorescence analysis of OCLN; white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (E). All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Journal: Journal of Cellular Physiology

    Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

    doi: 10.1002/jcp.70109

    Figure Lengend Snippet: Gene expression analysis of OCLN and markers related to its trafficking in WT and CB1 −/− (KO) testes. RTqPCR analysis of Ocln mRNA. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (A). Western blot analysis of OCLN. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD values (B). Immunofluorescence analysis of OCLN; white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN. Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene (E). All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Article Snippet: The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (see Figure ).

    Techniques: Gene Expression, Expressing, Western Blot, Immunofluorescence, Fluorescence, Imaging, Software

    Responsiveness of OCLN expression to CB1 and CB2 receptors. Western blot analysis of OCLN in testis of HT mice in vitro treated with vehicle (CTRL), ACEA (A) or JWH‐133 (B) at different concentrations: 0.1–1–10 µM. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD as fold change. Immunofluorescence analysis of OCLN in testis of CTRL and ACEA (10 µM) or JWH‐133 (1 µM); white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of Ocln mRNA in testis of CTRL and ACEA 10 µM (E) or JWH‐133 1 µM (F). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All the data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; a vs. b: p < 0.05).

    Journal: Journal of Cellular Physiology

    Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

    doi: 10.1002/jcp.70109

    Figure Lengend Snippet: Responsiveness of OCLN expression to CB1 and CB2 receptors. Western blot analysis of OCLN in testis of HT mice in vitro treated with vehicle (CTRL), ACEA (A) or JWH‐133 (B) at different concentrations: 0.1–1–10 µM. Protein amounts were quantified by densitometry analysis, normalized against ERK1/2 signals and expressed in OD as fold change. Immunofluorescence analysis of OCLN in testis of CTRL and ACEA (10 µM) or JWH‐133 (1 µM); white arrowheads represent OCLN localization (green) in testis. Nuclei were labelled with DAPI (blue). Scale bar: 5 µm (C). Quantitative analysis of OCLN green fluorescence on supra‐basal epithelium Regions of Interest (ROI); values are expressed as Sum of Intensity/area (SUM (I)/pixel 2 ), measured using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (D). RTqPCR analysis of Ocln mRNA in testis of CTRL and ACEA 10 µM (E) or JWH‐133 1 µM (F). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All the data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; a vs. b: p < 0.05).

    Article Snippet: The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (see Figure ).

    Techniques: Expressing, Western Blot, In Vitro, Immunofluorescence, Fluorescence, Imaging, Software

    CB1 and CB2 action on BTB permeability and functionality. FITC‐dextran diffusion assay in testis of WT and HT mice in vitro treated with vehicle (CTRL), ACEA 10 µM and JWH‐133 1 µM (A) Scale bar: 300 μm. The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) as SUM (I)/pixel 2 (see also Figure for technical details) (B) a versus b: p < 0.05. RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN in the testis of CTRL and ACEA 10 µM (C) or JWH‐133 1 µM (D). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Journal: Journal of Cellular Physiology

    Article Title: Cannabinoid Receptors Modulate Physiological Remodelling of the Blood–Testis Barrier

    doi: 10.1002/jcp.70109

    Figure Lengend Snippet: CB1 and CB2 action on BTB permeability and functionality. FITC‐dextran diffusion assay in testis of WT and HT mice in vitro treated with vehicle (CTRL), ACEA 10 µM and JWH‐133 1 µM (A) Scale bar: 300 μm. The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) as SUM (I)/pixel 2 (see also Figure for technical details) (B) a versus b: p < 0.05. RTqPCR analysis of endocytic (Eea1), recycling (Rab13) and degradation (Rab9 and Itch) markers of OCLN in the testis of CTRL and ACEA 10 µM (C) or JWH‐133 1 µM (D). Transcript amounts were reported as normalized fold expression (nfe) relatively to Rps18 gene. All data were reported as mean value ± S.E.M. (* p < 0.05; ** p < 0.01).

    Article Snippet: The green fluorescence signal diffused in the luminal compartment was quantified using the Nikon Imaging Analytical Software (NIS‐Elements Analysis D 4.40.00, 64 bit) (see Figure ).

    Techniques: Permeability, Diffusion-based Assay, In Vitro, Fluorescence, Imaging, Software, Expressing